Recombinant Neurotrophin 3 (NT3) Rattus norvegicus (Rat) Recombinant protein

NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Molecular Mass 15.9kDa, Accurate 18kDa(Analysis of differences refer to the manual)
  • Residues & TagsThr130~Gly256 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder, Purity > 90%
  • Isoelectric Point9.7
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  • Recombinant Neurotrophin 3 (NT3) Packages (Simulation)
  • Recombinant Neurotrophin 3 (NT3) Packages (Simulation)
  • RPA106Ra02.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • CaracterizaTeses: Disponiveis
  • Protective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factorpubmed:28388365
  • Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso:
  • Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered graftsPubmed: 31899965
  • Macrophage-derived neurotrophin-3 promotes heterotopic ossification in ratsPubmed: 31896816
  • Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­33389158
  • Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways33589896
  • Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism34092241
  • Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice34193676
  • Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons34712429
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377

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