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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Recombinant Signal Transducer And Activator Of Transcription 3 (STAT3)
APRF; Acute-Phase Response Factor
Organism species Homo sapiens (Human)
Product No.RPB743Hu01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass55.8kDa
Predicted isoelectric pointn/a
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular Location n/a
ResiduesArg335~Trp546 (Accession # P40763) with two N-terminal Tags, His-tag and GST-tag
FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- RPLVIK TGVQFTTKVR LLVKFPELNY QLKIKVCIDK DSGDVAALRG SRKFNILGTN TKVMNMEESN NGSLSAEFKH LTLREQRCGN GGRANCDASL IVTEELHLIT FETEVYHQGL KIDLETHSLP VVVISNICQM PNAWASILWY NMLTNNPKNV NFFTKPPIGT WDQVAEVLSW QFSSTTKRGL SIEQLTTLAE KLLGPGVNYS GCQITW
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAB743Hu22Monoclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)WB, ICC, IHC-P, IHC-F, ELISA
PAB743Hu01Polyclonal Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)WB, ICC, IHC-P, IHC-F, ELISA
PAB743Hu71Biotin-Linked Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)IHC;WB;ELISA
PAB743Hu81FITC-Linked Antibody to Signal Transducer And Activator Of Transcription 3 (STAT3)IHC;ICC;IF;WB
RPB743Hu01Recombinant Signal Transducer And Activator Of Transcription 3 (STAT3)SDS-PAGE; WB; ELISA; IP.
SEB743HuELISA Kit for Signal Transducer And Activator Of Transcription 3 (STAT3)ELISA