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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Recombinant Signal Transducer And Activator Of Transcription 4 (STAT4)
Organism species Homo sapiens (Human)
Product No.RPB739Hu01
SourceProkaryotic expression
HostE.coli
Purity> 95%
UOM50ug
Predicted Molecular Mass30.4kDa
Predicted isoelectric point7.3
ApplicationsSDS-PAGE; WB; ELISA; IP.
Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
Subcellular Location Cytoplasm. Nucleus
ResiduesTyr505~Ile737 (Accession # Q14765) with two N-terminal Tags, His-tag and T7-tag
FormulationSupplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- YVGRGL NSDQLHMLAE KLTVQSSYSD GHLTWAKFCK EHLPGKSFTF WTWLEAILDL IKKHILPLWI DGYVMGFVSK EKERLLLKDK MPGTFLLRFS ESHLGGITFT WVDHSESGEV RFHSVEPYNK GRLSALPFAD ILRDYKVIMA ENIPENPLKY LYPDIPKDKA FGKHYSSQPC EVSRPTERGD KGYVPSVFIP ISTIRSDSTE PHSPSDLLPM SPSVYAVLRE NLSPTTI
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAB739Hu22Monoclonal Antibody to Signal Transducer And Activator Of Transcription 4 (STAT4)WB, ICC, IHC-P, IHC-F, ELISA
PAB739Hu01Polyclonal Antibody to Signal Transducer And Activator Of Transcription 4 (STAT4)WB, ICC, IHC-P, IHC-F, ELISA
RPB739Hu01Recombinant Signal Transducer And Activator Of Transcription 4 (STAT4)SDS-PAGE; WB; ELISA; IP.
SEB739HuELISA Kit for Signal Transducer And Activator Of Transcription 4 (STAT4)ELISA