Recombinant Tumor Protein p53 (TP53)Homo sapiens (Human)Recombinant protein

p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • Product No.RPA928Hu01
  • Organism SpeciesHomo sapiens (Human)
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationNucleus, Mitochondrion, Cytoplasm, Endoplasmic reticulum lumen
  • Predicted Molecular Mass33.3kDa
  • Accurate Molecular Mass34kDa(Analysis of differences refer to the manual)
  • Residues & TagsGly108~Lys370 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • TraitsFreeze-dried powder
  • Purity> 97%
  • Isoelectric Point8.8
  • ApplicationsSDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
  • DownloadInstruction Manual
  • UOM10ug50ug250ug1mg1g
  • FOBUS$ 208US$ 520US$ 1300US$ 3120US$ 78000
  • Recombinant Tumor Protein p53 (TP53)Package and Components
  • Recombinant Tumor Protein p53 (TP53)Package and Components
  • Recombinant Tumor Protein p53 (TP53)Gene sequencing
  • RPA928Hu01.jpgSDS-PAGE
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

INCREMENT SERVICES

Reference

  • Anticancer ResearchAnalysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Microscopy Research and TechniqueTherapeutic role of curcumin in oxidative DNA damage caused by formaldehydePubMed: 25761397
  • J Nutr BiochemSupplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing micePubMed: 26344777