Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Rattus norvegicus (Rat) Recombinant protein

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

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Overview
Properties
  • Source Prokaryotic expression, Host E.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Cytoplasm
  • Molecular Mass 25.9kDa, Accurate 26kDa(Analysis of differences refer to the manual)
  • Residues & TagsGln2~Ala223 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder, Purity > 95%
  • Isoelectric Point5.6
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  • Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Packages (Simulation)
  • Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Packages (Simulation)
  • RPG945Ra01.jpg Figure. SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Sequence

Usage

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Citations

  • Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesionsPubMed: 26232084
  • Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcomePubmed:26810533
  • Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135
  • Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytespubmed:28667290
  • Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disabilityPubmed:29523900
  • Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injuryarticle:10.1088
  • Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhageISBN:978-952-03-0750-9
  • Association of neuronal repair biomarkers with delirium among survivors of critical illnessPubmed: 31896448
  • BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head TraumaPubmed: 32987792

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