ELISA Kit DIY Materials for Adiponectin (ADPN) Homo sapiens (Human) Assay Kit DIY Materials

GBP28; ApM1; AdipoQ; Acrp30; ACDC; APM1; C1Q And Collagen Domain Containing; Adipocyte Complement-Related Protein Of 30 KDa; Adipose Most Abundant Gene Transcript 1

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.069ng/mL.
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  • ELISA Kit DIY Materials for Adiponectin (ADPN) Packages (Simulation)
  • ELISA Kit DIY Materials for Adiponectin (ADPN) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Adiponectin (ADP).No significant cross-reactivity or interference between Adiponectin (ADP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Puerarin improves insulin resistance and modulates adipokine expression in rats fed a high-fat dietPubMed: 20869961
  • Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese RatsPubMed: PMC3591142
  • CD40L Deficiency Attenuates Diet-Induced Adipose Tissue Inflammation by Impairing Immune Cell Accumulation and Production of Pathogenic IgG-AntibodiesPubMed: PMC3297623
  • Soy isoflavones modulate adipokines and myokines to regulate lipid metabolism in adipose tissue, skeletal muscle and liver of male Huanjiang mini-pigsScienceDirect: S0303720712004194
  • n-6: n-3 PUFA ratio is involved in regulating lipid metabolism and inflammation in pigsPubmed: 23947577
  • Perirenal fat associated with microalbuminuria in obese ratsPubmed: 24526332
  • Maternal high folic acid supplement promotes glucose intolerance and insulin resistance in male mouse offspring fed a high-fat diet.Pubmed: 24736781
  • Adiponectin decreases lipids deposition by p38 MAPK/ATF2 signaling pathway in muscle of broilers.Pubmed: 24178343
  • CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytesPubmed:25168658
  • Zataria multiflora increases insulin sensitivity and PPARγ gene expression in high fructose fed insulin resistant ratsPubmed:Pmc4046234
  • Perinatal low-dose iron treatment influences susceptibility to diet-induced adipogenesis in early-aged male Wistar ratsPubmed:24519243
  • Effect of perches on liver health of hensPubmed:24812236
  • The effect of recombinant adeno-associated virus-adiponectin (rAAV2/1-Acrp30) on glycolipid dysmetabolism and liver morphology in diabetic ratsPubmed:25019654
  • Adipose tissue chromium and vanadium disbalance in high-fat fed Wistar ratsPubmed:25194956
  • Central alarin ameliorated insulin resistance of adipocytes in type 2 diabetic ratsPubmed:25240061
  • Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty RatsPubmed:25697126
  • Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathwayPubMed: 26617802
  • Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos PathwayPubMed: 26160485
  • Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew dietPubMed: 26188592
  • Relation between afamin level and some inflammatory markers in obese childrenMrjnrc: Abstract
  • Swimming training attenuates the morphological reorganization of the myocardium and local inflammation in the left ventricle of growing rats with untreated experimental diabetes.Pubmed:26896925
  • Decreased adipose tissue zinc content is associated with metabolic parameters in high fat fed Wistar ratsabstract:10
  • Changes on Adipose Tissue Distribution After Laparoscopic Roux-en-Y Gastric Bypass in Obese Göttingen Minipig. Effects on Glucose Metabolism.Pubmed:27185175
  • Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokinesscience:S0065128116301234
  • Trigonella foenum-graecum water extract improves insulin sensitivity and stimulates PPAR and γ gene expression in high fructose-fed insulin-resistant ratspmc:PMC4817393
  • Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Ratsarticle:s12011-016-0777-1
  • Antiobesity and antihyperlipidaemic effects of Yan-Sheng-Yin in animals and humansscience:S1756464616300585
  • Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult RatsPubmed:26175082
  • Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese micepubmed:27638193
  • Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adiposepubmed:27127795
  • Dietary Niacin Supplementation Suppressed Hepatic Lipid Accumulation in Rabbitspubmed:27004817
  • Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case- …vol10no1
  • Berberis integerrima ameliorates insulin resistance in high-fructose-fed insulin-resistant ratspubmed:29147484
  • BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathwayjournals:1758383
  • Differential Susceptibility of Germ and Leydig Cells to Cadmium-Mediated Toxicity: Impact on Testis Structure, Adiponectin Levels, and Steroidogenesisjournals:3405089
  • Antihyperglycemic Effect of Rosa Damascena is Mediated by PPAR.γ Gene Expression in Animal Model of Insulin Resistancepubmed:29201096
  • Association of ADIPOQ Gene Variant Rs266729 with Circulatory Adiponectin levels in Patients with type 2 Diabetes in North Indian Population: A Case-Control StudyDOI:10.13005/bpj/1123
  • FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathwaypubmed:28515350
  • Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD?pubmed:28499696
  • Circulating and Adipose Tissue mRNA Levels of Zinc-α2-Glycoprotein, Leptin, High-Molecular-Weight Adiponectin, and Tumor Necrosis Factor-Alpha in …Pubmed:29755407
  • Calycosin directly improves perivascular adipose tissue dysfunction by upregulating the adiponectin/AMPK/eNOS pathway in obese micePubmed:29595858
  • Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling …Pubmed:29619063
  • Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activityPubmed:29953010
  • Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemiaPubmed:30078593
  • Relationship between obesity and iron deficiency anemia: is there a role of hepcidin?Pubmed:29318945
  • Aging effects on circulating adiponectin levels and the expressions of adiponectin and adiponectin receptor 1 in the brains of male rats10.1016:j.ijge.2018.01.002
  • Epigallocatechin-3-gallate suppresses differentiation of adipocytes via regulating the phosphorylation of FOXO1 mediated by PI3K-AKT signaling in 3T3-L1 …Pubmed:29484120
  • Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic ratsarticle-1-352-en.pdf
  • Adiponectin may be used as a marker in prediction of diabetic retinopathy patientsJOCMS:Source
  • Combinational Effect of Pine Needle Polysaccharide and Kudzu Flavonoids on Cell Differentiation and Fat Metabolism in 3T3-L1 CellsDoi: 10.3136/fstr.24.903
  • Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndromePubmed: 30342783
  • Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat DietPubmed: 30400147
  • Effect of fermented food consumption on biochemical parameters and adipokines levelsDoi: 0.23751/pn.v20i4.7374
  • Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathwayPubmed: 30496353
  • Effects of Diet-Induced Early-Stage Obesity on a Low-Testosterone Gottingen Minipig
  • BuShenKangShuai Tablet Alleviates Hepatic Steatosis via Improving Liver Adiponectin Resistance in ApoE-/-Mice
  • Interrelation of liver vascularity to non-alcoholic fatty liver through a comparative study of the vasodilator effect of carvedilol or nicorandil in ratsPubmed: 30826497
  • Cardiac Lymphatic Dysfunction Causes Drug-Eluting Stent–Induced Coronary Hyperconstricting Responses in Pigs In VivoPubmed: 30816801
  • Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP …Pubmed: 31003008
  • Expression of adipokines and their receptors in adipose tissue of women with class 3 obesity with or without hypertensionPubmed: 30940525
  • 冷暴露对高脂饮食小鼠脂肪棕色化的影响
  • High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
  • Paternal chronic folate supplementation induced the transgenerational inheritance of acquired developmental and metabolic changes in chickensPubmed: 31506054
  • Increased plasma osteopontin levels are associated with nonalcoholic fatty liver disease in patients with type 2 diabetes mellitusPubmed: 31514105
  • Collaborative Effects of Chlorogenic Acid and Caffeine on Lipid Metabolism via AMPKα-LXRα/SREBP-1c Pathway in High-Fat Diet-Induced Obese MicePubmed: 31670366
  • Dietary supplementation of Morus nigra L. leaves decrease fat mass partially through elevating leptin-stimulated lipolysis in pig modelPubmed: 31756448
  • Serum Asprosin Concentrations Are Increased and Associated with Insulin Resistance in Children with ObesityPubmed: 31775140
  • The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese childrenPubmed: 31771561
  • Triphenyl phosphate causes a sexually dimorphic metabolism dysfunction associated with disordered adiponectin receptors in pubertal micePubmed: 31796355
  • HOMA-Adiponectin Closely Associates with Cardiometabolic Risk Markers in Middle-Aged Indians with Metabolic SyndromePubmed: 32189318
  • Cerasus humilis cherry polyphenol reduces high-fat diet-induced obesity in C57BL/6 mice by mitigating fat deposition, inflammation, and oxidationPubmed: 32227855
  • AdipoR1/AdipoR2 dual agonist recovers nonalcoholic steatohepatitis and related fibrosis via endoplasmic reticulum-mitochondria axisPubmed: 33199780
  • Adipocytokines and Metabolic Syndrome in Patients with SchizophreniaPubmed: 33066473
  • Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with ObesityPubmed: 33027789
  • Resemblance and differences in dietary restriction nephroprotective mechanisms in young and old ratsPubmed: 32970613
  • Serum ZAG and Adiponectin Levels Were Closely Related to Obesity and the Metabolically Abnormal Phenotype in Chinese PopulationPubmed: 32904655
  • Adiponectin treatment improves insulin resistance in mice by regulating the expression of the mitochondrial-derived peptide MOTS-c and its response to exercise via …Pubmed: 32880686
  • Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with …Pubmed: 32816247
  • Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced ObesityPubmed: 32633953
  • Activation of central galanin receptor 2 mitigated insulin resistance in adipocytes of diabetic ratsPubmed: 32588381
  • The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patientsPubmed: 32569480
  • Exercise-Induced Increases in Insulin Sensitivity After Bariatric surgery are Mediated by Muscle Extracellular Matrix RemodelingPubmed: 32409493
  • Cooperative effects of galanin and leptin on alleviation of insulin resistance in adipose tissue of diabetic ratsPubmed: 32395890
  • Study of serum adiponectin and resistin in patients with obstructive sleep apnea
  • Association of+ 10211T/G (Rs17846866) Variant of Adiponectin Gene With Type 2 Diabetes Mellitus
  • In-silico therapeutic investigations of arjunic acid and arjungenin as an FXR agonist and validation in 3T3-L1 adipocytes
  • The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren33575861
  • Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren33834274
  • TMBIM1 is an inhibitor of adipogenesis and its depletion promotes adipocyte hyperplasia and improves obesity-related metabolic disease34107313
  • Decreased Plasma MANF Levels are Associated with Type 2 Diabetes33766221
  • Effect of Ileal Transposition (IT) on Angiopoietin-Like Protein-8 (ANGPTL8) and Pentraxin (PTX3) Plasma Level in Sprague-Dawley Rats Fed High-Fat Diet?¡­34035808
  • Anti-Obesity Efficacy of Selected Plants Against Progesterone Induced Obesity
  • Smilax china L. polyphenols alleviates obesity and inflammation by modulating gut microbiota in high fat/high sucrose diet-fed C57BL/6J mice
  • Altered Serum Marker of Adipokines Profile in Breast Cancer Women
  • Activation of the Peroxisome Proliferator-Activated Receptors (PPAR-α/γ) and the Fatty Acid Metabolizing Enzyme Protein CPT1A by Camel Milk Treatment …34306045
  • The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study34348809
  • Smilax china L. flavonoid alleviates HFHS-induced inflammation by regulating the gut-liver axis in mice34561124
  • Vitamin D3 enhances the effects of omega‐3 oils against metabolic dysfunction‐associated fatty liver disease in rat34767670
  • Associations of imbalance of intestinal flora with severity of disease, inflammatory factors, adiponectin, and vascular endothelial function of hypertension patients34672426
  • GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity34853313
  • Effects of Obesity on Adiponectin System Skin Expression in Dogs: A Comparative Study34438765
  • Hepatic deficiency of selenoprotein S exacerbates hepatic steatosis and insulin resistancePubmed:35347118
  • Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in IndiaPubmed:35717896
  • Adiponectin Levels Are Associated with White Matter Lesions (WMLs) and Cognitive ImpairmentPubmed:35087911
  • High Circulating Fibroblast Growth Factor-21 Levels as a Prognostic Marker in Fatty Pancreas Patients
  • SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.

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