ELISA Kit DIY Materials for Annexin A1 (ANXA1) Homo sapiens (Human) Assay Kit DIY Materials

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.117ng/mL
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  • ELISA Kit DIY Materials for Annexin A1 (ANXA1) Packages (Simulation)
  • ELISA Kit DIY Materials for Annexin A1 (ANXA1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Annexin A1 (ANXA1).No significant cross-reactivity or interference between Annexin A1 (ANXA1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns DiseasePubMed: PMC3794972
  • Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2Ajol: Source
  • Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic ResponseAsm: Source
  • Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)PubMed: 26718731
  • Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet FunctionPubMed: 26470781
  • Annexin A1 Is Increased in the Plasma of Preeclamptic WomenPubMed: 26398190
  • 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义article:8686
  • Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.pubmed:28062503
  • Mesenchymal stromal cells improve human islet function through released products and extracellular matrix.pubmed:29101297
  • Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesityPubmed:29677533
  • Annexin A1 is elevated in patients with COPD and affects lung fibroblast functionPubmed:29440885
  • Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patientsPubmed:29370415
  • EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT …pdf:3395
  • Resolution of inflammation in neuromyelitis optica spectrum disordersPubmed: 30300851
  • Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines
  • Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolitesPubmed: 32133417
  • ANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathwayPubmed: 32908567
  • Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients33263684
  • Annexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK¦Á stability via selective autophagy33523920

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