ELISA Kit DIY Materials for Cystatin C (Cys-C) Homo sapiens (Human) Assay Kit DIY Materials

CST3; Cystatin 3; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.67ng/mL
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  • ELISA Kit DIY Materials for Cystatin C (Cys-C) Packages (Simulation)
  • ELISA Kit DIY Materials for Cystatin C (Cys-C) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Cystatin 3 (CST3). No significant cross-reactivity or interference between Cystatin 3 (CST3) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

  • The Renal Effects of Vanadate Exposure: Potential Biomarkers and Oxidative Stress as a Mechanism of Functional Renal Disorders—Preliminary StudiesHindawi: 740105
  • Endothelial dysfunction and renal fibrosis in endotoxemia-induced oliguric kidney injury: possible role of LPS-binding proteinPubmed:25261195
  • РОЛЬ АПОПТОЗ ИНДУЦИРУЮЩЕГО ФАКТОРА И ОКИСЛИТЕЛЬНОГО СТРЕССА ПРИ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ616-005.8
  • Protective Effects of DHA-PC against Vancomycin-Induced Nephrotoxicity through the Inhibition of Oxidative Stress and Apoptosis in BALB/c Micepubmed:29254330
  • Association of cathepsin B and cystatin C with an age-related pulmonary subclinical state in a healthy Chinese populationPubmed: 32401159
  • SERS based Y-shaped aptasensor for early diagnosis of acute kidney injuryPubmed:35733690
  • A Compared Study of Gentle Ketogenic Diet Containing Medium-Chain Triglycerides or Long-Chain Triglycerides on Chronic Sleep Deprivation-Induced Cognitive …Pubmed:35141738

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