ELISA Kit DIY Materials for Isocitrate Dehydrogenase 1, Soluble (IDH1)

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,260.00 US$ 2,100.00 US$ 3,780.00 US$ 7,350.00 US$ 12,600.00
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Overview

Properties

Product No.KSH839Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryEnzyme & Kinase

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.781-50ng/mL
Applicable Sensitivity0.37ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Isocitrate Dehydrogenase 1, Soluble (IDH1). No significant cross-reactivity or interference between Isocitrate Dehydrogenase 1, Soluble (IDH1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

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ELISA / CLIA Experiment Service

Increment services

Citations

Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546
Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanismPubmed:29921847
IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancerPubmed:29465809
Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver …Pubmed: 32443439
Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancerPubmed:35689436