ELISA Kit DIY Materials for Matrix Metalloproteinase 9 (MMP9) Homo sapiens (Human) Assay Kit DIY Materials

GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.059ng/mL.
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  • ELISA Kit DIY Materials for Matrix Metalloproteinase 9 (MMP9) Packages (Simulation)
  • ELISA Kit DIY Materials for Matrix Metalloproteinase 9 (MMP9) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9).No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c micePubMed: 21119180
  • Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal HyperplasiaScienceDirect: S0022480409001942
  • The Biological Behaviors of Rat Dermal Fibroblasts Can Be Inhibited by High Levels of MMP9ACS: pr2006395
  • The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary MenZjrms: Source
  • Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental strokePubMed: 21679435
  • Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Hypothermia induced by adenosine 5′-monophosphate attenuates early stage injury in an acute gouty arthritis rat modelPubmed: 23408150
  • The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cellsPubmed: 23544611
  • RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cellsPubmed: 24681113
  • Expression and inhibition of matrix metalloproteinase (MMP)-8, MMP-9 and MMP-12 in early colonic anastomotic repairPubmed: 23619615
  • Effects of Photodynamic Therapy on the Clinical and Gingival Crevicular Fluid Inflammatory Biomarkers in Chronic Periodontitis: A Split-Mouth Randomized Clinical TrialJoponline: 130464
  • Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative coursePubmed: 23585206
  • Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
  • Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis modelPubmed:Pmc4132129
  • Pass-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in ratsPubmed:24701154
  • Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasionPubmed:25078863
  • Changes in biological behaviors of rat dermal fibroblasts induced by high expression of MMP9Pubmed:25215164
  • Melissa officinalis Extract Inhibits Laser-Induced Choroidal Neovascularization in a Rat ModelPubmed:25314292
  • Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In VitroPubmed:25767513
  • A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary MenPhypha:Source
  • Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the HorseJournals: Mi
  • MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1Content: Asp
  • Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by ParaquatPubMed: 25945502
  • In vivoPubMed: 25634132
  • Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cellsPubMed: 26254457
  • Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathwaysPubMed: 26191138
  • Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cellsPubMed: 25884474
  • Melanocortin MC5 receptor agonists alleviate brain damage in abdominal compartment syndrome in the ratPubMed: 25616531
  • Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosisPubMed: 26412140
  • Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in ratsDoi: Abs
  • A comparative study to evaluate the effect of limited access dressing on diabetic ulcersArticle: 2347-9264
  • Влияние диуретиков с антиальдостероновым действием на состояние межклеточного матрикса и раннее постинфарктное ремоделирование левого …Article: View
  • матриксу та раннArticle: Viewfile
  • Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγPubMed: 26703570
  • ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial CellsPubmed:27075970
  • CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthmaJournal:27699
  • Chronic myelogenous leukaemia exosomes modulate bone marrow microenvironment through activation of epidermal growth factor receptorPubmed:27196940
  • Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic RatsPubmed:26851971
  • Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cellsPubmed:26991449
  • MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1Pubmed:27160777
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
  • sequential measurements of serum matrix metalloproteinase 9 to monitor chemotherapy responses in patients with advanced non-small-cell lung cancerpmc:PMC4898419
  • Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.Pubmed:26963343
  • Gallium, a promising candidate to disrupt the vicious cycle driving osteolytic metastases.pubmed:27378505
  • Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases.pubmed:28348451
  • Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damagepubmed:27761275
  • The effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic …pubmed:27652141
  • Increased Plasma Matrix Metalloproteinase-9 Levels Contribute to Intracerebral Hemorrhage during Thrombolysis after Concomitant Stroke and Influenza Infectionpubmed:27560521
  • Pleiotropic effects of survivin in vascular endothelial cells.pubmed:27373518
  • Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction.pubmed:27938355
  • MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1.pubmed:27160777
  • MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Linepublication:306067745
  • Actein protects against methylglyoxal-induced oxidative damage in osteoblastic MC3T3-E1 cells.pubmed:26991449
  • The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female.pubmed:28174048
  • Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblastsart00008
  • Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activitypubmed:28091828
  • MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1pubmed:27743351
  • Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosispubmed:28821422
  • MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1pubmed:28573504
  • The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cellspubmed:29224019
  • Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
  • Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9DOI:10.1111/fcp.12294
  • Alantolactone induces apoptosis, promotes STAT3 glutathionylation and enhances chemosensitivity of A549 lung adenocarcinoma cells to doxorubicin via oxidative stressdoi:10.1038/s41598-017-06535-y
  • Amphiregulin contained in NSCLC-exosomes induces osteoclast differentiation through the activation of EGFR pathwaypubmed:28600504
  • CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma.pubmed:28507585
  • Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritispubmed:28901438
  • A Time Course of Bevacizumab (Anti-VEGF) Effect on Rat Peritoneum: Relations Between Antiadhesive Action and Fibrin Regulation Enzymes.pubmed:28877644
  • Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodelingPubmed:29572844
  • Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia …10.1002:jcb.26849
  • Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral NeuropathyPubmed:29704151
  • sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasionPubmed:30022839
  • CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular RemodelingPubmed:29984229
  • miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells10.3892:ol.2018.9170
  • HMGB1-RAGE signaling pathway in pPROMPubmed:29673663
  • Clinical assessment after human adipose stem cell transplantation into dogsPubmed:29284215
  • Inhibition of SRC/FAK cue: A novel pathway for the synergistic effect of rosuvastatin on the anti-cancer effect of dasatinib in hepatocellular carcinomaPubmed: 30292831
  • Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF.Pubmed: 30464333
  • Targeting peptidylarginine deiminase reduces neutrophil extracellular trap formation and tissue injury in severe acute pancreatitisPubmed: 30515825
  • A prospective, randomized trial of intravenous hydroxocobalamin versus norepinephrine or saline for treatment of lipopolysaccharide‐induced hypotension in a swine …Pubmed: 30575111
  • Evaluation of the Effects of Biohcly in an Model of Mechanical Wounds in the Rabbit CorneaPubmed: 30888249
  • Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and OsteoblastsPubmed: 30838809
  • MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1
  • Histopathological and Biochemical Evaluation of the effect of Paeoniflorin on the Periodontium During and After Periodontitis Formation in RatsPubmed: 31005686
  • МАТРИКСНЫЕ МЕТАЛЛОПРОТЕИНАЗЫ: ЗНАЧЕНИЕ В РЕМОДЕЛИРОВАНИИ СЕРДЦА У ПАЦИЕНТОВ С ДИСПЛАЗИЕЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ
  • Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma
  • Expression of MMP-9 and TIMP-1 in rat models of pressure ulcer and their significance
  • Hydroxytyrosol Supplementation Modifies Plasma Levels of Tissue Inhibitor of Metallopeptidase 1 in Women with Breast CancerPubmed: 31514476
  • The levels of matrix metalloproteinase-9 and neutrophil gelatinase-associated lipocalin in different stages of endometriosisPubmed: 31177884
  • Evaluation of the anti-atherogenic potential of Egyptian artichoke leaf extract in hypercholesterolemic ratsPubmed: 31566004
  • Proportion of the CD19-Positive and CD19-Negative Lymphocytes and Monocytes within the Peripheral Blood Mononuclear Cell Set Is Characteristic for Rheumatoid …Pubmed: 31554310
  • MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16Pubmed: 31796150
  • Plasma matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 as prognostic biomarkers in critically ill patientsPubmed: 32190734
  • Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosisPubmed: 31980722
  • Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertensionPubmed: 33175289
  • TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK …Pubmed: 33068589
  • Multiple Myeloma-Derived Extracellular Vesicles Induce Osteoclastogenesis through the Activation of the XBP1/IRE1α AxisPubmed: 32759820
  • Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitisPubmed: 32709888
  • Biomarkers Facilitate the Assessment of Prognosis in Critically Ill Patients with Primary Brain Injury: A Cohort StudyPubmed: 32575870
  • A novel role of VEPH1 in regulating AoSMC phenotypic switchingPubmed: 32342520
  • Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairmentPubmed: 28593326
  • Intracranial Aneurysm Biomarker Candidates Identified by a Proteome-Wide StudyPubmed: 32525733
  • Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats
  • Anti-arthritic Effects of Platelets Rich Plasma and Hyaluronic Acid on Adjuvant-induced Arthritis in Rats
  • Evaluation of altered ground matrix and matrix metalloproeinase (MMP'S) in wound healing with Aloe vera extract
  • Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep
  • Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats
  • ДИНАМИКА МАРКЕРА ФИБРОЗИРОВАНИЯ ММР-9 У БОЛЬНЫХ ЛИМФОМОЙ ХОДЖКИНА
  • Krill oil and low-dose aspirin combination mitigates experimentally induced silicosis in rats: role of NF-¦ÊB/TGF-¦Â1/MMP-9 pathway33398741
  • GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression33613720
  • Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes33491733
  • Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population33850223
  • Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays34012227
  • Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia33381317
  • The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin?¡­33381190
  • Intermittent Pacing Therapy Favorably Modulates Infarct Remodeling28386775
  • Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline …34167392
  • Time-resolved Fluorescence Immunoassay (TRFIA) for the Simultaneous Detection of MMP-9 and Lp-PLA2 in Serum34495467
  • Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke34591349
  • Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer34650982
  • Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study34707724
  • Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot StudyPubmed:35743980
  • Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary StudyPubmed:35625637
  • Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid …
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
  • Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes

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