ELISA Kit DIY Materials for Aquaporin 1 (AQP1) Mus musculus (Mouse) Assay Kit DIY Materials

AQP-CHIP; CHIP28; CO; Channel-Forming Integral Protein,28kDa; Aquaporin-CHIP; Urine water channel; Water channel protein for red blood cells and kidney proximal tubule

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.070ng/mL
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  • ELISA Kit DIY Materials for Aquaporin 1 (AQP1) Packages (Simulation)
  • ELISA Kit DIY Materials for Aquaporin 1 (AQP1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Aquaporin 1, Colton Blood Group (AQP1). No significant cross-reactivity or interference between Aquaporin 1, Colton Blood Group (AQP1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patientsScienceDirect: S0304394011012341
  • Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertensionPubmed:26853804
  • Cerebrospinal fluid levels of tumor necrosis factor alpha and aquaporin 1 in patients with mild cognitive impairment and idiopathic normal pressure hydrocephalusPubmed:27155076
  • Neuron-derived Plasma Exosome Proteins after Remote Traumatic Brain InjuryPubmed: 31441374
  • Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus34597351

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