ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF) Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse) Assay Kit DIY Materials

Neurotrophin; Abrineurin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity12.6pg/mL
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  • ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • ELISA Kit DIY Materials for Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Interaction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat BrainsPubMed: 21436126
  • Neuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cordScienceDirect: S0022510X12001219
  • Effect of Benthosemapterotum Peptide on Aging in Mice with Oxidative DamagePsrcentre: 212059
  • Chronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone miceScienceDirect: S0304394010015788
  • Neuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.NCBI: PMC3925622
  • The effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factorPubmed:25446810
  • Heme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord.Pubmed:26559686
  • Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young ratspmc:PMC4812310
  • Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • Effect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3
  • Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.pubmed:27914953
  • PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice.pubmed:27940378
  • Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalitiespubmed:28081639
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Long‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic …bdi.12449
  • Associations of blood parameters with age, feed efficiency and sampling routine in young beef bullsscience:article
  • Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen–induced allergic rhinitispubmed:28412394
  • A new comorbidity model and the common pathological mechanisms of migraine and epilepsypubmed:28559979
  • Alpha-asarone attenuates depression-like behavior in nicotine-withdrawn mice: Evidence for the modulation of hippocampal pCREB levels during nicotine-withdrawal.pubmed:29042206
  • An enriched environment restores hepatitis B vaccination-mediated impairments in synaptic function through IFN-γ/Arginase1 signalingPubmed:29627530
  • Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse modelPubmed:29335016
  • N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotypePubmed:29203442
  • Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemiaPubmed:30012511
  • Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model.Pubmed:29890692
  • Delta-9-Tetrahydrocannabinol (∆-THC) Induce Neurogenesis and Improve Cognitive Performances of Male Sprague Dawley RatsPubmed:28933048
  • Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in ratsPubmed:29522778
  • Long term Westernized diet leads to region-specific changes in brain signaling mechanismsPubmed:29655945
  • Changes in exercise capacity and serum BDNF following long-term sprint interval training in well-trained cyclistsPubmed: 30286300
  • Establishment and evaluation of a novel mouse model of peri/postmenopausal depressionDoi: 10.1016/j.heliyon.2019.e01195
  • Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling
  • Effect of voluntary running on expression of myokines in brains of rats with depressionPubmed: 30834799
  • Clinical usefulness of brain-derived neurotrophic factor and visinin-like protein-1 in early diagnostic tests for acute strokePubmed: 30826210
  • Monoaminergic system is implicated in the antidepressant-like effect of hyperoside and protocatechuic acid isolated from Impatiens glandulifera Royle in micePubmed: 31077758
  • Physical Exercise Increased Brain-Derived Neurotrophic Factor in Elderly Population with DepressionPubmed: 30994834
  • Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer's Disease Model MicePubmed: 31561359
  • Cognitive Decline of Rats with Chronic Fluorosis Is Associated with Alterations in Hippocampal Calpain SignalingPubmed: 31797207
  • Chronic harmine treatment has a delayed effect on mobility in control and socially defeated ratsPubmed: 32088835
  • Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicityPubmed: 32010279
  • Association of neuronal repair biomarkers with delirium among survivors of critical illnessPubmed: 31896448
  • CSF levels of a set of neurotrophic factors (brain-derived neurotrophic factor, nerve growth factor) and neuropeptides (neuropeptide Y, galanin) in epileptic childrenPubmed: 32327377
  • Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and …Pubmed: 32918393
  • Impaired LTD-like motor cortical plasticity in female patients with major depression disorderPubmed: 32791084
  • Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's diseasePubmed: 32777662
  • Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
  • Comparison of serum brain-derived neurotrophic factor (BDNF) in dogs with and without separation anxiety
  • Nicotinamide riboside alleviates alcohol-induced depression-like behaviours in C57BL/6J mice by altering the intestinal microbiota associated with microglial …
  • Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine
  • Blood Plasma Trophic Growth Factors Predict the Outcome in Patients with Acute Ischemic Stroke
  • Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi
  • Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­33389158
  • Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways33589896
  • Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 …34239303
  • Tropomyosin receptor kinase B-mediated signaling in integration of neuropathic pain and obesity in diabetic polyneuropathy34586159
  • Moderate-intensity continuous training and high-intensity interval training improve cognition, and BDNF levels of middle-aged overweight men34762211
  • Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression34824208
  • Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons34712429
  • Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid …34384732
  • CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway34296452
  • Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader …Pubmed:35098494
  • Gender influence on clinical manifestations, depressive symptoms and brain-derived neurotrophic factor (BDNF) serum levels in patients affected by …Pubmed:35344113
  • Associations between serum levels of brain-derived neurotrophic factor, corticotropin releasing hormone and mental distress in vitiligo patientsPubmed:35508633
  • Co-Delivery of Docosahexaenoic Acid and Brain-Derived Neurotropic Factor from Electrospun Aligned Core–Shell Fibrous Membranes in Treatment of Spinal Cord …Pubmed:35214053
  • Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …Pubmed:35585377
  • Effect of herbal formulation Sharbat Ahmad Shahi on serum BDNF level in mild to moderate cases of depressive disorder
  • Medicinal effects of Saffron on obese animals by reducing Castellis risk index to protect from cardio-pathology and psychological distress

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