ELISA Kit DIY Materials for Follistatin Like Protein 1 (FSTL1) Mus musculus (Mouse) Assay Kit DIY Materials

FRP; FSL1; Follistatin-related protein 1

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity5.8pg/mL
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  • ELISA Kit DIY Materials for Follistatin Like Protein 1 (FSTL1) Packages (Simulation)
  • ELISA Kit DIY Materials for Follistatin Like Protein 1 (FSTL1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Follistatin Like Protein 1 (FSTL1). No significant cross-reactivity or interference between Follistatin Like Protein 1 (FSTL1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Serum S100A8/A9, but not follistatin-like protein 1 and interleukin 18, may be a useful biomarker of disease activity in adult-onset Still's disease.PubMed: 22660800
  • Regulation of follistatin-like protein 1 expression and secretion in primary human skeletal muscle cellsPubmed: 23419164
  • Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferationPubmed:25118038
  • Growth Differentiation Factor-15 (GDF-15) Levels Are Associated with Cardiac and Renal Injury in Patients Undergoing Coronary Artery Bypass Grafting with Cardiopulmonary BypassPubmed:25171167
  • Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistancePubMed: 26199111
  • Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Menpmc:PMC4856269
  • Follistatin like 1 Regulates Hypertrophy in Heart Failure with Preserved Ejection Fraction.pubmed:27430031
  • Follistatin like-1 aggravates silica-induced mouse lung injuryPMC5428474
  • Plasma follistatin-like protein 1 is correlated with disease severity in patients with acute pulmonary embolism and predicts short-term mortalityISSN:1936-2625
  • High circulating follistatin-like protein 1 as a biomarker of a metabolically unhealthy statePubmed: 30745500
  • Associations among circulating levels of follistatin-like 1, clinical parameters, and cardiovascular events in patients undergoing elective percutaneous …Pubmed: 31034503
  • Improved early detection of ovarian cancer using longitudinal multimarker modelsPubmed: 31937926
  • Targeting Follistatin like 1 ameliorates liver fibrosis induced by carbon tetrachloride through TGF-β1-miR29a in micePubmed: 32933544
  • Effects of acute endurance exercise on follistatin-like 1 and apelin in the circulation and metabolic organs in ratsPubmed: 32412800
  • Prognostic impact of transcardiac gradient of follistatin-like 1 reflecting hemodynamics in patients with dilated cardiomyopathy34334268
  • Association between Plasma Follistatin-like Protein 1 Levels and the Presence and Severity of Coronary Artery Disease34853217

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