ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE) Homo sapiens (Human) Assay Kit DIY Materials

Insulysin; Insulin Protease; Abeta-degrading protease; Insulinase

  • Product No.KSB897Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.59ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
  • Quantity
  • Add to Cart Distributors
  • ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE) Packages (Simulation)
  • ELISA Kit DIY Materials for Insulin Degrading Enzyme (IDE) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Degrading Enzyme (IDE). No significant cross-reactivity or interference between Insulin Degrading Enzyme (IDE) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

  • J NeurochemReductionPubMed: 26442993
  • African Journal of Pharmacy and Pharmacology Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic ratsarticle-full-text-pdf

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