ELISA Kit DIY Materials for Interleukin 15 (IL15)

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,361.00 US$ 2,269.00 US$ 4,084.00 US$ 7,942.00 US$ 13,614.00
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Overview

Properties

Product No.KSA061Ga01
Organism SpeciesChicken (Gallus) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
Downloadn/a
CategoryCytokine Infection immunity

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range15.6-1,000pg/mL
Applicable Sensitivity6.2pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Interleukin 15 (IL15). No significant cross-reactivity or interference between Interleukin 15 (IL15) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

Increment services

Citations

Expression of interleukin-15 and inflammatory cytokines in skeletal muscles of STZ-induced diabetic rats: effect of resistance exercise trainingPubmed: 24006180
Time course of IL-15 expression after acute resistance exercise in trained rats: effect of diabetes and skeletal muscle phenotypePubmed:25522723
miR-15b is Downregulated in Myasthenia Gravis Patients and Directly Regulates the Expression of Interleukin-15 (IL-15) in Experimental Myasthenia Gravis MicePubMed: 26087886
Effects of intrinsic aerobic capacity, aging and physical activity on interleukin-15 protein level in serum and skeletal muscleDspace: Handle
Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mices10529-017-2342-1
Interleukin-15 and cisplatin co-encapsulated thermosensitive polypeptide hydrogels for combined immuno-chemotherapy.pubmed:28408199