ELISA Kit DIY Materials for Lysyl Oxidase (LOX) Homo sapiens (Human) Assay Kit DIY Materials

Protein-Lysine 6-Oxidase

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.061ng/mL
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  • ELISA Kit DIY Materials for Lysyl Oxidase (LOX) Packages (Simulation)
  • ELISA Kit DIY Materials for Lysyl Oxidase (LOX) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Lysyl Oxidase (LOX). No significant cross-reactivity or interference between Lysyl Oxidase (LOX) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms.PubMed: 23494965
  • High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 CellsPubmed: 22966818
  • Evaluation of serum lysyl oxidase as a blood test for colorectal cancer Pubmed: 24246612
  • Copia autorizada por CDRIngentaconnect: Source
  • Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosisPubmed: 24574233
  • Evaluation of serum lysyl oxidase as a blood test for colorectal cancerPubmed:Pmc4237001
  • Ionizing radiation induces tumor cell lysyl oxidase secretionPubmed:Pmc4223762
  • Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical CancerPubmed:24945327
  • Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic SclerosisPubMed: 26095613
  • Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-ß Levels and LOXL1 Genotype in PseudoexfoliationPubmed:27116380
  • LOX Fails to Substitute for RANKL in Osteoclastogenesis.pubmed:27606829
  • Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases.pubmed:28445728
  • Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritispubmed:28901438
  • A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection FractionPubmed:29807761
  • Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitroPubmed:29393489
  • Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressivenessPubmed: 30398411
  • Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis.Pubmed: 33026236
  • Collagen Cross©\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy33356379

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