ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10) Homo sapiens (Human) Assay Kit DIY Materials

STMY2; SL2; Stromelysin 2; Transin-2

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range78.12-5000pg/mL
  • Applicable Sensitivity34pg/mL
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  • ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10) Packages (Simulation)
  • ELISA Kit DIY Materials for Matrix Metalloproteinase 10 (MMP10) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 10 (MMP10). No significant cross-reactivity or interference between Matrix Metalloproteinase 10 (MMP10) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Type-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodellingWiley: source
  • Matrix Metalloproteinase-10 Is Required for Lung Cancer Stem Cell Maintenance, Tumor Initiation and Metastatic PotentialPubMed: PMC3335833
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic strokePubmed:24671655
  • Plasma inflammatory biomarkers for Huntington’s disease patients and mouse model Pubmed:25266150
  • In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative studyPubmed:25634132
  • Plasma inflammatory biomarkers for Huntington’s disease patients and mouse modelPubMed: 25266150

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