ELISA Kit DIY Materials for Monocyte Chemotactic Protein 3 (MCP3) Homo sapiens (Human) Assay Kit DIY Materials

CCL7; FIC; MARC; NC28; MCP3; SCYA7; Chemokine C-C-Motif Ligand 7; Small Inducible Cytokine A7; Monocyte Chemoattractant Protein 3

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.62-1000pg/mL
  • Applicable Sensitivity5.8pg/mL
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  • ELISA Kit DIY Materials for Monocyte Chemotactic Protein 3 (MCP3) Packages (Simulation)
  • ELISA Kit DIY Materials for Monocyte Chemotactic Protein 3 (MCP3) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 3 (MCP3). No significant cross-reactivity or interference between Monocyte Chemotactic Protein 3 (MCP3) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effect of omega 3 fatty acids plus low-dose aspirin on both clinical and biochemical profiles of patients with chronic periodontitis and type 2 diabetes: a randomized double blind placebo-controlled studyPubmed:25604769
  • Epigallocatechin-3-Gallate Attenuates Unilateral Ureteral Obstruction-Induced Renal Interstitial Fibrosis in MicePubMed: 25549657
  • MicroRNA-155 Promotes Atherosclerosis Inflammation via Targeting SOCS1PubMed: 26159489
  • Ex vivo Pretreatment of Islets with Mitomycin C: Reduction in Immunogenic Potential of Islets by Suppressing Secretion of Multiple Chemotactic Factors.pubmed:28901184
  • Molybdenum disulfide/graphene oxide nanocomposites show favorable lung targeting and enhanced drug loading/tumor-killing efficacy with improved …Articles:am2017225
  • Immunomodulation by adoptive regulatory T-cell transfer improves Coxsackievirus B3-induced myocarditisPubmed:29863913

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