ELISA Kit DIY Materials for Mucin 1 (MUC1) Mus musculus (Mouse) Assay Kit DIY Materials

CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; CA153; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.054ng/mL
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  • ELISA Kit DIY Materials for Mucin 1 (MUC1) Packages (Simulation)
  • ELISA Kit DIY Materials for Mucin 1 (MUC1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 1 (MUC1). No significant cross-reactivity or interference between Mucin 1 (MUC1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
  • Host Defense Proteins in Breast Milk and Neonatal Yeast ColonizationPubMed: 26116637
  • Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive IndicatorPubMed: 26446295
  • The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital FluidsPubmed:27182834
  • The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghaifiles:ijcem0020163.pdf
  • Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification.pubmed:28107977
  • An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
  • Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA10.21769:BioProtoc.2879
  • Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review
  • A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample

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