ELISA Kit DIY Materials for Serotonin Transporter (SERT) Mus musculus (Mouse) Assay Kit DIY Materials

SLC6A4; SLC6-A4; HTT; 5-HTT; 5HTT; OCD1; hSERT; Solute Carrier Family 6,Member 4; Neurotransmitter Transporter,Serotonin; Sodium-dependent serotonin transporter

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.055ng/mL
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  • ELISA Kit DIY Materials for Serotonin Transporter (SERT) Packages (Simulation)
  • ELISA Kit DIY Materials for Serotonin Transporter (SERT) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Serotonin Transporter (SERT). No significant cross-reactivity or interference between Serotonin Transporter (SERT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Comparison of platelet serotonin transporter activity in subjects with severe sleep bruxism and controlPubmed:25127373
  • Experimental heart failure causes depression-like behavior together with differential regulation of inflammatory and structural genes in the brainPubmed:25400562
  • Sleep bruxism frequency and platelet serotonin transporter activities in young adult subjectsPubMed: 26527205
  • Non-Serotonergic Neurotoxicity by MDMA (Ecstasy) in Neurons Derived fromMouse P19 Embryonal Carcinoma Cells.pubmed:27861613
  • Herz-Hirn Interaktion im Mausmodell: Herzinsuffizienz nach Myokardinfarkt führt zu depressivem Verhalten bei Mäusenfrontdoor:index
  • Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Ratsarticle_1486_c0fa9f8afe5f70b407e7195bcfd55c70
  • Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor …Pubmed:29854627
  • Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles
  • Effects of regulating gut microbiota on the serotonin metabolism in the chronic unpredictable mild stress rat modelPubmed: 31323174
  • VAMP8 and serotonin transporter levels are associated with venous thrombosis risk in a Spanish female population. Results from the RETROVE ProjectPubmed: 31382081
  • Neuronal Cell Death Induces Depressive Disorder in Rats Depression-Like Behaviors Caused by Chronic Stress
  • Administration of a Probiotic Mixture Ameliorates Cisplatin-Induced Mucositis and Pica by Regulating 5-HT in Rats34568500
  • Targeting the oxytocin system to ameliorate early life depressive-like behaviors in maternally-separated rats34349049

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