ELISA Kit DIY Materials for Tumor Protein, Translationally Controlled 1 (TPT1) Mus musculus (Mouse) Assay Kit DIY Materials

HRF; TCTP; p02; p23; Fortilin; Translationally-Controlled Tumor Protein; Histamine-releasing factor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.119ng/mL
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  • ELISA Kit DIY Materials for Tumor Protein, Translationally Controlled 1 (TPT1) Packages (Simulation)
  • ELISA Kit DIY Materials for Tumor Protein, Translationally Controlled 1 (TPT1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Tumor Protein, Translationally Controlled 1 (TPT1). No significant cross-reactivity or interference between Tumor Protein, Translationally Controlled 1 (TPT1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Translationally controlled tumour protein is associated with podocyte hypertrophy in a mouse model of type 1 diabetesSpringerLink: e14361700w260x75
  • Synergistic Actions of Histamine-Releasing Factor and Histamine Releasing Factor-Reactive IgE in Chronic Urticaria.pubmed:28219065
  • dTBP2 attenuates severe airway inflammation by blocking inflammatory cellular network mediated by dTCTP34628164

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