Mouse Model for Tumor Transplantation (TT) Mus musculus (Mouse) Disease model

Transplanted tumors;TE-1;HepG2;HepG2-luc;SK-MES-1;LL/2-luc-M38

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by HepG2-luc in situ tumor formation
  • Model Animal StrainsBalb/ -nude Mice(SPF), healthy, male, age:5 weeks, body weight:18g~20g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high).
  • Modeling Period4-6 weeks
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  • Mouse Model for Tumor Transplantation (TT) Packages (Simulation)
  • Mouse Model for Tumor Transplantation (TT) Packages (Simulation)
  • Mouse Model for Tumor Transplantation (TT) Fig. The liver ( the arrow:tumor on the liver) of nude mice, HepG2-luc cells were injected in situ for 4 weeks
  • DSI530Mu02.jpg Fig. Fluorescence in vivo imaging after HepG2-luc cells were injected in situ for 1 w
  • Mouse Model for Tumor Transplantation (TT) Fig. Fluorescence in vivo imaging after HepG2-luc cells were injected in situ for 1 w
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1. Cell line: HepG2-luc human hepatoma cell line
2. Liver tumor in situ formation model :
2.1 The HepG2-luc cells cultured in logarithmic growth phase were digested and centrifuged, and the resulting approximately 5 x 10e6 cells were dissolved in 0.1 mL DMEM culture medium.
2.2 Use 3% pentobarbital to anesthetize mice, laparotomy to expose the liver, the prepared cells were injected into the liver of nude mice, with 5-0 silk suture wounds, povidone iodine to disinfect wounds, the nude mice were placed in a heating pad, stay awake after normal diet feeding.
3. Fluorescence in vivo imaging:
For in vivo fluorescence imaging detection of tumor in nude mice, mice were anesthetized with 3% pentobarbital sodium used before imaging, by intraperitoneal injection of 3mg luciferin substrate, the substrate after the injection of 20~30 minutes after the in vivo fluorescence imaging detection.

Model evaluation

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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