CLIA Kit for Annexin A1 (ANXA1) Homo sapiens (Human) Sandwich CLIA

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

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  • CLIA Kit for Annexin A1 (ANXA1) Packages (Simulation)
  • CLIA Kit for Annexin A1 (ANXA1) Packages (Simulation)
  • CLIA Kit for Annexin A1 (ANXA1) Results demonstration
  • SCE787Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Annexin A1 (ANXA1) and the recovery rates were calculated by comparing the measured value to the expected amount of Annexin A1 (ANXA1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-103 89
EDTA plasma(n=5) 93-104 101
heparin plasma(n=5) 91-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Annexin A1 (ANXA1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Annexin A1 (ANXA1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 97-105% 90-99% 87-96%
EDTA plasma(n=5) 93-101% 78-98% 82-98% 93-102%
heparin plasma(n=5) 91-98% 78-93% 79-102% 93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Annexin A1 (ANXA1)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Annexin A1 (ANXA1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Annexin A1 (ANXA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Annexin A1 (ANXA1) level in the sample or standard.;

Citations

  • Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns DiseasePubMed: PMC3794972
  • Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2Ajol: Source
  • Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic ResponseAsm: Source
  • Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)PubMed: 26718731
  • Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet FunctionPubMed: 26470781
  • Annexin A1 Is Increased in the Plasma of Preeclamptic WomenPubMed: 26398190
  • 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义article:8686
  • Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.pubmed:28062503

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