Rat Model for Dystonia (DT)

1. Modeling and identification of dystonia in rats
1.1 Modeling method
The experiment was conducted after adaptive feeding of male SD rats for a week .After anesthesia, the rats in the model group were fixed on the stereotaxic instrument. The coordinates of the left caudate putamen were determined to be 1mm of bregma, 3mm on the left side of the sagittal suture, and ~7mm of dura mater. Routine disinfection was performed, the skin and subcutaneous tissues of the head were cut, the outer membrane of the skull was bluntly separated, the anterior fontanelle and the left skull were fully exposed, and the skull was drilled open (be careful not to damage the dura). 3-NP solution (1000nmol/ul) was injected into the target with a microsyringe, 4ul per injection. 4~7 points were injected subdural, and the needle was left for 5 minutes after the injection, and the needle was withdrawn slowly. The control group was injected with normal saline at the same site. The animals were routinely fed in the same environment after operation.

1.Flat score:
Before modeling, all animals were trained on a flat: a narrow flat, 120cm long and 7cm wide, was placed between two platforms 1m high from the ground. All rats were trained 3 times a day for 7 days to ensure that each rat could successfully pass the flat plate without stopping in between. The time and number of steps taken by each rat through the tablet were recorded to calculate stride length.
Five days after the operation, animals in each group were subjected to the flat test, and the time and number of steps of each rat were recorded to calculate the stride length.

2.Motor coordination test:
The motor coordination ability of rats was quantified by rotarod test.The rats' stay time on the rotating rod was tested at 9r/min and 18r/min respectively. Each animal was measured three times at each rotating speed and the average value was taken.
3.Take sample and detection:
The rats were sacrificed 7 days after operation for brain tissue and serum samples.

HE staining and Nissl staining:
Brain tissue was taken, embedded and sected, and HE staining and Nissl staining were performed to observe pathological changes, mainly observing the changes of neurons in striatum, midbrain and hippocampus.

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.