CLIA Kit for Vitamin B1 (VB1) 
Thiamine; Thiamin; Aneurine
- UOM
- FOB US$ 571.00 US$ 816.00 US$ 3,672.00 US$ 6,936.00 US$ 57,120.00
- Quantity
Overview
Properties
- Product No.CCD053Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- ApplicationsChemiluminescent immunoassay for Antigen Detection.
Research use only - DownloadInstruction Manual
- Category
Sign into your account
Share a new citation as an author
Upload your experimental result
Review
Contact us
Please fill in the blank.
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Recovery
Matrices listed below were spiked with certain level of Vitamin B1 (VB1) and the recovery rates were calculated by comparing the measured value to the expected amount of Vitamin B1 (VB1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 94-104 | 99 |
| heparin plasma(n=5) | 97-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin B1 (VB1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin B1 (VB1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B1 (VB1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 95-104% | 87-101% | 80-101% | 91-99% |
| EDTA plasma(n=5) | 93-105% | 87-95% | 88-99% | 94-105% |
| heparin plasma(n=5) | 99-105% | 80-99% | 92-105% | 94-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| Substrate A | 1×10mL | Substrate B | 1×2mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
Test principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Vitamin B1 (VB1). A competitive inhibition reaction is launched between biotin labeled Vitamin B1 (VB1) and unlabeled Vitamin B1 (VB1) (Standards or samples) with the pre-coated antibody specific to Vitamin B1 (VB1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin B1 (VB1) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Vitamin B1 (VB1) level in the sample or standard.
Giveaways
Increment services
Citations
- Identification of critical variants within SLC44A4, an ulcerative colitis susceptibility gene identified in a GWAS in north IndiansPubmed:26741288
- 日粮不同钴水平对奶牛泌乳性能, 营养物质消化率和血液生化指标的影响
- Disinhibition-Like Behavior Correlates with Frontal Cortex Damage in an Animal Model of Chronic Alcohol Consumption and Thiamine DeficiencyPubmed:35203470