Anti-Extracellular Signal Regulated Kinase 1 (ERK1) Polyclonal Antibody

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 107.00 US$ 251.00 US$ 358.00 US$ 895.00 US$ 3,580.00
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Overview

Properties

Product No.CAB357Mi01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsLoading Control of WB.
Research use only
DownloadInstruction Manual
CategorySignal transduction Enzyme & Kinase Apoptosis Tumor immunity

PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
Packages (Simulation)

Figure Western Blot Sample: Recombinant ERK1, Human
Figure Western Blot
Lane1: Human A549 Cells
Lane2: Human Lung Tissue
Primary Ab: 1:300 Dilution of Rabbit Anti-Human ERK1 Ab
Second Ab: 1:5000 Dilution of HRP-Linked Rabbit
Anti-Mouse IgG Ab (Catalog: SAA544Mu09)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 1:150-600
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndromepubmed:27538909
Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis33575317
Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026