Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody

G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 107.00 US$ 251.00 US$ 358.00 US$ 895.00 US$ 3,580.00
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Overview

Properties

Product No.CAB932Mi22
Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsLoading Control of WB
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Metabolic pathway Cardiovascular biology Hepatology Nutrition metabolism

PurificationProtein A + Protein G affinity chromatography
LabelNone
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)

Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate Primary Ab: 1µg/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Porcine Cerebrum lysate; Lane4: Bovine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Equine Liver lysate; Lane7: Caprine Cerebrum lysate
Primary Ab: 3µg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot analysis of GAPDH in various cell lines
Lane1: DU145 whole cell lysate
Lane2: OS-RC-2 whole cell lysate
Lane3: T-47D whole cell lysate
Lane4: HEC-1B whole cell lysate
Lane5: HepG2 whole cell lysate
Lane6: HEK-293 whole cell lysate
Lysates/proteins at 20µg per lane.
Primary Ab: anti-GAPDH antibody (CAB932Mi22) at 0.1ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique.
Calculated MW: 36KD
Observed MW: 36KD
Exposure time: 1 min
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Liver lysate; Lane4: Cavia Kidney lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Liver lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Caprine Liver lysate; Lane10: Mouse Liver lysate; Lane11: Rat Liver lysate; Lane12: Rat Kidney lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.

Usage

Enzyme-Linked Immune Sorbent Assay:
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Hamster (Chinese hamster), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

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Citations

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Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
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Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipidsPubmed: 30237167
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Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood CellsPubmed: 30488202
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Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathwayPubmed: 31078923
Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid LeukemiaPubmed: 31718075
Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor …Pubmed: 32046779
Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathwayPubmed: 32028241
Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in ratsPubmed: 33170419
Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo
Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice
BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 58133540760
Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity33262291
Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026
Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysisPubmed:34981672
Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegenerationPubmed:35151792
Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiencyPubmed:35577991