Anti-Proliferating Cell Nuclear Antigen (PCNA) Monoclonal Antibody

Cyclin

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 119.00 US$ 279.00 US$ 398.00 US$ 995.00 US$ 3,980.00
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Overview

Properties

Product No.CAA591Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsLoading Control of WB.
Research use only
DownloadInstruction Manual
CategoryTumor immunity Developmental science

PurificationProtein A + Protein G affinity chromatography
LabelNone
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Thyroid cancer Tissue; Primary Ab: 20µg/ml Mouse Anti-Human PCNA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Endometrial cancer Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20ug/ml Mouse Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Colon Tissue;
Primary Ab: 30ug/ml Mouse Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 30ug/ml Mouse Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 30ug/ml Mouse Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 30µg/ml Mouse Anti-Human PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 30µg/ml Mouse Anti-Human PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 30µg/ml Mouse Anti-Human PCNA Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 0.75μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)

Figure. Western Blot; Sample: Recombinant PCNA, Human.
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Porcine Kidney lysate; Lane3: Canine Lung lysate; Lane4: Bovine Testis lysate; Lane5: Caprine Lung lysate; Lane6: Cavia Lung lysate; Lane7: Equine Liver lysate
Primary Ab: 1µg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 3µg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human A431 cell lysate;
Primary Ab: 3µg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 3µg/ml Mouse Anti-Human PCNA Antibody
Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against PCNA. It has been selected for its ability to recognize PCNA in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.0003-0.1ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse)

Giveaways

Increment services

Citations

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