Monoclonal Antibody to Annexin V (ANXA5)

ANX5; ENX2; PP4; PAP-I; CBP-I; Anchorin CII; Calphobindin I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; Thromboplastin inhibitor; Vascular anticoagulant-alpha

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 101.00 US$ 235.00 US$ 336.00 US$ 840.00 US$ 3,360.00
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Overview

Properties

Product No.MAA259Hu22
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryApoptosis Hematology

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG1 Kappa, Clone Number C13
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPA259Hu01-Recombinant Annexin V (ANXA5)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 40µg/ml Mouse Anti-Human ANXA5 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 40µg/ml Mouse Anti-Human ANXA5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 40µg/ml Mouse Anti-Human ANXA5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 40µg/ml Mouse Anti-Human ANXA5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Placenta Tissue;
Primary Ab: 40µg/ml Mouse Anti-Human ANXA5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 20µg/ml Mouse Anti-Human ANXA5 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue
Primary Ab: 20µg/ml Mouse Anti-Human ANXA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Small intestine Tissue
Primary Ab: 20µg/ml Mouse Anti-Human ANXA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Mouse Anti-Human ANXA5 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Figure.Western Blot;Sample:Recombinant ANXA5, Human
Western Blot; Sample: Lane1: Human Placenta lysate; Lane2: Hela cell lysate; Lane3: Porcine Lung lysate; Lane4: Bovine Testis lysate
Primary Ab: 0.2μg/ml Mouse Anti-Human ANXA5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Canine Lung lysate; Lane2: Equine Lung lysate; Lane3: Rabbit Lung lysate; Lane4: Cavia Lung lysate; Lane5: Ovine Lung lysate
Primary Ab: 0.2μg/ml Mouse Anti-Human ANXA5 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ANXA5. It has been selected for its ability to recognize ANXA5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-4µg/mL;
Immunohistochemistry: 2-40µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Cavia (Guinea pig ), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse)

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Citations

Markers of apoptotic dysfunctions in schizophreniaPubmed: 24466757
Involvement of Anomalous Apoptosis in Impairments to Synaptic Plasticity in Post-Traumatic Stress DisorderSpringer: Source
Collectins, C3 complement protein, annexin V and C-reactive protein in acute ischemic stroke: interrelation and implication to upregulated apoptosis and inflammationSmartscitech: Source
Annexin A5 Protein as a Potential Biomarker for the Diagnosis of AsthmaPubmed: 30182154