Polyclonal Antibody to N-cadherin (NCAD) Rattus norvegicus (Rat) Polyclonal antibody

CDw325; CD325; CDHN; CDH2; Cadherin 2 Type 1; N-Cadherin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB481Ra03-Recombinant N-cadherin (NCAD)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to N-cadherin (NCAD) Packages (Simulation)
  • Polyclonal Antibody to N-cadherin (NCAD) Packages (Simulation)
  • Polyclonal Antibody to N-cadherin (NCAD) IHC Images
  • PAB481Ra03.jpg Figure. Western Blot; Sample: Recombinant CDH2, Rat.
  • Polyclonal Antibody to N-cadherin (NCAD) Western Blot; Samples: Lane1: Mouse Heart lysate; Lane2: Rat Cerebrum lysate;
    Primary Ab: 0.2µg/ml Rabbit Anti-Rat NCAD Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against NCAD. It has been selected for its ability to recognize NCAD in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

Giveaways

Citations

  • Biomarkers for methotrexate-induced liver injury: urinary protein profiling of psoriasis patients.Pubmed: 23830989
  • Circulating N-cadherin levels are a negative prognostic indicator in patients with multiple myeloma.Pubmed: 23438504
  • Elevated Cerebrospinal Fluid and Plasma N-Cadherin in Alzheimer DiseasePubmed: 32296844
  • ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer33566221
  • Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence33758996
  • DOC2B is a Negative Regulator of Wnt/β-catenin Signaling Pathway in Cervical CancerPubmed:35500882

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