Polyclonal Antibody to Caspase 1 (CASP1) Rattus norvegicus (Rat) Polyclonal antibody

ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB592Ra01-Recombinant Caspase 1 (CASP1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Caspase 1 (CASP1) Packages (Simulation)
  • Polyclonal Antibody to Caspase 1 (CASP1) Packages (Simulation)
  • Polyclonal Antibody to Caspase 1 (CASP1) DAB staining on IHC-P; Samples: Rat Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat CASP1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 1 (CASP1) DAB staining on IHC-P;
    Samples: Rat Kidney Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Rat CASP1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB592Ra01.jpg WB Image
  • Polyclonal Antibody to Caspase 1 (CASP1) Western Blot; Sample: Human Serum;
    Primary Ab: 1µg/ml Rabbit Anti-Rat CASP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 1 (CASP1) Western Blot; Sample: Mouse Serum;
    Primary Ab: 1µg/ml Rabbit Anti-Rat CASP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 1 (CASP1) Western Blot; Sample: Rat Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Rat CASP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 1 (CASP1) Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Rat Cerebrum lysate
    Primary Ab: 0.2μg/ml Rabbit Anti-Rat CASP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 1 (CASP1) Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Mouse Spleen lysate
    Primary Ab: 0.5μg/ml Rabbit Anti-Rat CASP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CASP1. It has been selected for its ability to recognize CASP1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

Giveaways

Citations

  • Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-ReperfusionPubmed:24816434
  • Inflammosome in the human endometrium: further step in the evaluation of the “maternal side”PubMed: 26474737
  • Synthetic PreImplantation Factor (PIF) prevents fetal loss by modulating LPS induced inflammatory response.pubmed:28704412
  • NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitinationPubmed:29386291
  • Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation?Pubmed:29665864
  • A role for the inflammasome in spontaneous labor at termPubmed:27852921
  • CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSESPubmed: 29386291
  • NLRP3 inflammasome expression in lipopolysaccharide-induced otitis mediaPubmed: 30676848
  • Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss womenPubmed: 31148259
  • Serum Caspase-1 levels in women with polycystic ovary syndromePubmed: 32127139
  • Increased Level of Caspase-1 in the Serum of Relapsing-remitting Multiple Sclerosis (RRMS) Patients
  • ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES
  • CLINICAL AND PATHOGENETIC SIGNIFICANCE OF CHANGES IN PURINE METABOLISM DURING LUNG CANCER
  • The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation34560082
  • The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice34632484
  • TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion InjuryPubmed:35041191
  • Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network DispersionPubmed:35062292

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