Polyclonal Antibody to Caspase 4 (CASP4) Bos taurus; Bovine (Cattle) Polyclonal antibody

ICE(rel)II; ICH2; TX; Protease ICH-2; Cysteinyl Aspartate Specific Proteinases 4; Apoptosis-Related Cysteine Peptidase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB735Bo01-Recombinant Caspase 4 (CASP4)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Caspase 4 (CASP4) Packages (Simulation)
  • Polyclonal Antibody to Caspase 4 (CASP4) Packages (Simulation)
  • PAB735Bo01.jpg Figure. Western Blot; Sample: Recombinant CASP4, Bovine.
  • Polyclonal Antibody to Caspase 4 (CASP4) Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Rat Liver lysate; Lane3: Cavia Liver lysate; Lane4: Equine Liver lysate
    Primary Ab: 0.2μg/ml Rabbit Anti-Bovine CASP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19) Selected
  • Polyclonal Antibody to Caspase 4 (CASP4) Western Blot; Sample: Bovine Serum;
    Primary Ab: 5µg/ml Rabbit Anti-Bovine CASP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 4 (CASP4) Western Blot; Sample: Lane1: Bovine Liver lysate; Lane2: Bovine Stomach lysate; Lane3: Bovine Colon lysate; Lane4: Mouse Liver lysate
    Primary Ab: 1.5μg/ml Rabbit Anti-Bovine CASP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 4 (CASP4) Western Blot; Sample: Lane1: THP1 cell lysate; Lane2: U937 cell lysate
    Primary Ab: 1.5μg/ml Rabbit Anti-Bovine CASP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 4 (CASP4) Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Rat Liver lysate; Lane3: Porcine Liver lysate; Lane4: Canine Liver lysate; Lane5: Gallus Small intestine lysate; Lane6: Cavia Liver lysate; Lane7: Equine Liver lysate
    Primary Ab: 1.5μg/ml Rabbit Anti-Bovine CASP4 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CASP4. It has been selected for its ability to recognize CASP4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Equus caballus; Equine (Horse), Chicken (Gallus)

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