Polyclonal Antibody to Caspase 7 (CASP7) Rattus norvegicus (Rat) Polyclonal antibody

CMH1; ICE-LAP3; MCH3; Caspase 7 Apoptosis-Related Cysteine Peptidase; Cysteine Aspartate Protease 7; Cysteinyl Aspartate Specific Proteinases 7

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA449Ra01-Recombinant Caspase 7 (CASP7)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Caspase 7 (CASP7) Packages (Simulation)
  • Polyclonal Antibody to Caspase 7 (CASP7) Packages (Simulation)
  • Polyclonal Antibody to Caspase 7 (CASP7) DAB staining on IHC-P; Samples: Rat Stomach Tissue; Primary Ab: 20µg/ml Rabbit Anti-Rat CASP7 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 7 (CASP7) DAB staining on IHC-P;
    Sample: Rat Stomach Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Rat CASP7 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Caspase 7 (CASP7) FITC staining on IF;
    Samples: Mouse 3T3-L1 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Rat CASP7 Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Caspase 7 (CASP7) FITC staining on IF;
    Sample: 3T3-L1 cell
    Primary Ab: 20µg/ml Rabbit Anti-Rat CASP7 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA449Ra01.jpg WB Image
  • Polyclonal Antibody to Caspase 7 (CASP7) Western Blot; Sample: Lane1: Mouse Lung lysate; Lane2: 3T3-L1 cell lysate
    Primary Ab: 0.3μg/ml Rabbit Anti-Rat CASP7 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CASP7. It has been selected for its ability to recognize CASP7 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

Giveaways

Citations

  • Protective Effects of Morus alba Leaves Extract on Ocular Functions of Pups from Diabetic and Hypercholesterolemic Mother RatsPubMed: 21697998
  • Analysis of fine structure and biochemical changes of retina during aging of Wistar albino ratsOnlinelibrary: ceo.12123
  • Aging-related changes of optic nerve of Wistar albino ratsPubmed:Pmc4039266
  • Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritisPubmed:25511416
  • Inhibition of small HA fragment activity and stimulation of A2APubMed: 25511416
  • Effects of elevated body temperature on structure and function of cerebrum and cerebellum of Wistar male albino ratsPdf-Files: Issue-4-2015
  • Role of Pomegranate Juice and Atorvastatin in Ameliorating Spinal Neurotoxicity of Wistar Rats Maternally Fed on Hypercholesterolemic DietDOI: 10.4172/2155-9929.1000316
  • Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2InhibitorsPubmed: 30191744
  • Synergistic effects of pomegranate juice and atorvastatin for improving cerebellar structure and function of breast-feeding rats maternally fed on a high cholesterol dietPubmed: 32339653
  • Fish-oil supplementation improves retinal injury induced by diabetes and hypercholesterolemia in male Wistar rats

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