KO-Validated
Polyclonal Antibody to Collagen Type III Alpha 1 (COL3a1)

COL3-A1; COL3A1; EDS4A; Collagen Type III Alpha 1; Ehlers-Danlos Syndrome Type IV Autosomal Dominant; Collagen Alpha-1(III)chain

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAD195Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryMetabolic pathway

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD195Hu01-Recombinant Collagen Type III Alpha 1 (COL3a1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

IHC Images

Figure. Western Blot; Sample: Recombinant COL3a1, Human.
Western Blot; Sample: Porcine Spleen lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human COL3a1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: Porcine Spleen lysate; Lane2: BXPC3 cell lysate; Lane3: Hela cell lysate; Lane4: RAW264.7 cell lysate;
Primary Ab: 0.3µg/ml Rabbit Anti-Human COL3a1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type BXPC3 cell lysate;
Lane 2: COL3a1 knockout BXPC3 cell lysate;
Predicted MW: 111,138kd
Observed MW: 110kd
Primary Ab: 1µg/ml Rabbit Anti-Human COL3a1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against COL3a1. It has been selected for its ability to recognize COL3a1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Increment services

Citations

Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-cultureUnivmed:Source
Epithelial but not stromal expression of collagen alpha-1 (III) is a diagnostic and prognostic indicator of colorectal carcinomaPubmed:26741506
Association between Plasma HMGB-1 and Silicosis: A Case-Control StudyPubmed: 30558126

KO-Validated Polyclonal Antibody to Collagen Type III Alpha 1 (COL3a1)