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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Creatine Kinase, Muscle (CKM)
CK-MM; CKMM; CK3; CK-M; MM-CK
Organism species Homo sapiens (Human)
Product No.PAA109Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant CKM (Lys11~Leu367) expressed in E.coli.
Accession No.: RPA109Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM VEMEKKL
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CKM. It has been selected for its ability to recognize CKM in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA109Hu22Monoclonal Antibody to Creatine Kinase, Muscle (CKM)WB, ICC, IHC-P, IHC-F, ELISA
PAA109Hu01Polyclonal Antibody to Creatine Kinase, Muscle (CKM)WB, ICC, IHC-P, IHC-F, ELISA
PAA109Hu71Biotin-Linked Antibody to Creatine Kinase, Muscle (CKM)IHC;WB;ELISA
RPA109Hu01Recombinant Creatine Kinase, Muscle (CKM)SDS-PAGE; WB; ELISA; IP.
SEA109HuELISA Kit for Creatine Kinase, Muscle (CKM)Enzyme-linked immunosorbent assay