Polyclonal Antibody to Cyclophilin A (CYPA) Homo sapiens (Human) Polyclonal antibody

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA979Hu01-Recombinant Cyclophilin A (CYPA)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Cyclophilin A (CYPA) Packages (Simulation)
  • Polyclonal Antibody to Cyclophilin A (CYPA) Packages (Simulation)
  • Polyclonal Antibody to Cyclophilin A (CYPA) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CYPA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human CYPA Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) DAB staining on IHC-P;
    Sample: Porcine Testis Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human CYPA Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA979Hu01.jpg Figure. Western Blot; Sample: Recombinant CYPA, Human.
  • Polyclonal Antibody to Cyclophilin A (CYPA) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 4µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) Western Blot; Sample: Human Serum;
    Primary Ab: 4µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) Western Blot; Sample: Rat Serum;
    Primary Ab: 4µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: HepG2 cell lysate; Lane4: Jurkat cell lysate
    Primary Ab: 0.05µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) Western Blot; Samples: Lane1: Mouse Testis lysate; Lane2: Mouse Cerebrum lysate; Lane3: Rat Lung lysate; Lane4: Hela cell lysate;
    Primary Ab: 0.1µg/ml Rabbit Anti-Human CYPA Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cyclophilin A (CYPA) Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human CYPA Polyclonal Antibody (Catalog PAA979Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CYPA. It has been selected for its ability to recognize CYPA in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formationPubMed: 19758589
  • Cyclophilin-A: a novel biomarker for untreated male essential hypertensionPubmed: 24164594
  • Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular diseasePubmed:24502618
  • Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial InfarctionPubmed:25552928
  • Cyclophilin A in Ruptured Intracranial AneurysmPubMed: 26426668
  • PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery diseasePubMed: 26702934
  • Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes MellitusPubMed: 26496315
  • Cyclophilin A modulates bone marrow-derived CD117(+) cells and enhances ischemia-induced angiogenesisvia the SDF-1/CXCR4 axis.Pubmed:27057951
  • Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitorpubmed:27823952
  • Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus.pubmed:27809851
  • Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activityPubmed:29382697
  • Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitisPubmed:29318411
  • Cyclophilin A and CD147 associate with progression of diabetic nephropathyPubmed: 30572748
  • Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarctionPubmed: 31670477
  • Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome PatientsPubmed: 31936351
  • APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers …Pubmed: 24030206
  • Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy
  • Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy34437430
  • Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell deathPubmed:35732625

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