Polyclonal Antibody to Cytochrome P450 1A2 (CYP1A2)

CP12; P3-450; P450(PA); Cytochrome P450,Family 1,Subfamily A,Polypeptide 2

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 114.00 US$ 266.00 US$ 380.00 US$ 950.00 US$ 3,800.00
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Overview

Properties

Product No.PAD294Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryMetabolic pathway

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPD294Ra01-Recombinant Cytochrome P450 1A2 (CYP1A2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 500µg/mL

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Packages (Simulation)
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Figure: FITC staining on IHC-P; Sample: Hela cells.
Figure: FITC staining on IHC-P;
Sample: Hela cells.

Figure. Western Blot; Sample: Recombinant CYP1A2, Rat.
Figure. Western Blot; Sample: Lane1: Rat Liver Tissue; Lane2: Human Hela Cells; Lane3: Mouse Liver Tissue.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CYP1A2. It has been selected for its ability to recognize CYP1A2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

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Citations

Differential expression of HO-1 and CYP1A2 during up-regulation of ERK in stressed fish hepatocytesPubmed:25471622
Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4PubMed: 25733458
Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar ratsPubmed:27133590
Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats.pubmed:27133590
Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stressb0407ebde042c4f61b728bc11a586e6c
Liver lobe and strain differences in the activity of murine cytochrome p450 enzymesPubmed:29879457
Stammesspezifische Unterschiede in der analgetischen Wirkung von Buprenorphin bei der Maus