Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) Homo sapiens (Human) Monoclonal antibody

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a Kappa, Clone Number C3
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB357Hu01-Recombinant Extracellular Signal Regulated Kinase 1 (ERK1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) Packages (Simulation)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) Packages (Simulation)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) DAB staining on IHC-P; Sample: Human Ovarian cancer Tissue; Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) DAB staining on IHC-P;
    Sample: Human Kidney Tissue;
    Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) DAB staining on IHC-P;
    Sample: Human Colon Tissue;
    Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) DAB staining on IHC-P;
    Sample: Human Liver Tissue;
    Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1) DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue;
    Primary Ab: 20μg/ml Mouse Anti-Human ERK1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB357Hu22.jpg Figure. Western Blot; Sample: Recombinant ERK1, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndromepubmed:27538909
  • Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis33575317
  • Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026

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