Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) Mus musculus (Mouse) Polyclonal antibody

GSHPX1; Cellular glutathione peroxidase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA295Mu01-Recombinant Glutathione Peroxidase 1 (GPX1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) Packages (Simulation)
  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) Packages (Simulation)
  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) DAB staining on IHC-P;
    Samples: Mouse Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) DAB staining on IHC-P;
    Samples: Mouse Spleen Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) DAB staining on IHC-P;
    Sample: Mouse Liver Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glutathione Peroxidase 1 (GPX1) DAB staining on IHC-P;
    Sample: Mouse Spleen Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse GPX1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA295Mu01.jpg Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Blood Cells lysate Primary Ab: 1µg/ml Rabbit Anti-Mouse GPX1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GPX1. It has been selected for its ability to recognize GPX1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine SupplementationPlos:Source
  • Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closurePubmed:24443098
  • Determination of Gene Expression and Serum Levels of MnSOD and GPX1 in Colorectal CancerPubmed:25550558
  • Genetic polymorphisms (Pro197Leu of Gpx1, +35A/C of SOD1, PubMed: 26674569
  • Seasonal Variation in Glutathione Peroxidase in Seminal Plasma of Karan Fries (Tharparkar X Holstein Friesian) Bulls Under Tropical Climatic Conditionspublication:301246301
  • Influence of season on seminal antioxidant enzymes in Karan Fries bulls under tropical climatic conditionsfileID:697741
  • Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanismpubmed:27599793
  • Reduced Dietary Selenium Impairs Vascular Function by Increasing Oxidative Stress in Sprague-Dawley Rat Aortas.pubmed:28574428
  • Assessing the serum concentration levels of NT-4/5, GPX-1, TNF-α, and l-arginine as biomediators of depression severity in first depressive episode patients with and without posttraumatic stress disorder.pubmed:28958613
  • Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot studypubmed:29248027
  • Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stresspubmed:28654832
  • Selenium- Essential Antioxidant Elemen the example of autoimune thyroiditispdf:44%20PREDA%20C%207%2017.pdf
  • Seasonal variation of mitochondria activity related and heat shock protein genes in spermatozoa of Karan Fries bulls in tropical climate10.1080:09291016.2017.1361584
  • Investigation of GPX1 Gene Expression, Serum GPX1 and Selenium Levels on Colorectal Cancer
  • Kolon Kanserinde Gpx1 Gen Anlatımı, Serum Gpx1 ve Selenyum Düzeylerinin İncelenmesi
  • Influence of deficient nutrition on trace element status and antioxidant defense systemPubmed: 1781925
  • Glutathione, glutathione peroxidase and some hematological parameters of HIV-seropositive subjects attending clinic in University of Calabar teaching …Pubmed: 31703562
  • Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
  • AMELIORATIVE EFFECT OF AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA (ROSELLE) ON SALT-INDUCED HYPERTENSION IN WISTAR RATS
  • In Vitro Cell Culture Study of Anticholesterol and Antioxidant Activities of Myrmecodia platytyrae (MyP) Extract in WRL-68 Cells
  • Effects of Ganoderma lucidum polysaccharides on different pathways involved in the development of spinal cord ischemia reperfusion injury: biochemical ¡­33689849
  • Associations of Antioxidant Enzymes with the Concentration of Fatty Acids in the Blood of Men with Coronary Artery Atherosclerosis34945751
  • Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver diseasePubmed:35086715
  • A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …Pubmed:35281603

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