Polyclonal Antibody to Hemopexin (HPX) Homo sapiens (Human) Polyclonal antibody

Haemopexin; Beta-1B-glycoprotein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Hemopexin (HPX) Packages (Simulation)
  • Polyclonal Antibody to Hemopexin (HPX) Packages (Simulation)
  • Polyclonal Antibody to Hemopexin (HPX) DAB staining on IHC-P; Sample: Human Placenta Tissue Primary Ab: 10µg/ml Rabbit Anti-Human HPX Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hemopexin (HPX) DAB staining on IHC-P;
    Sample: Porcine Liver Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human HPX Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hemopexin (HPX) DAB staining on IHC-P;
    Sample: Porcine Lung Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human HPX Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB986Hu02.jpg Figure. Western Blot; Sample: Recombinant HPX, Human.
  • Polyclonal Antibody to Hemopexin (HPX) Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Liver lysate; Lane3: Human Placenta lysate; Lane4: Human Serum
    Primary Ab: 1µg/mL Rabbit Anti-Human HPX Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Hemopexin (HPX) Western Blot; Sample: Rat Plasma
    Primary Ab: 0.01µg/ml Rabbit Anti-Human HPX Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HPX. It has been selected for its ability to recognize HPX in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Semi-quantitative measurement of a specific glycoform using a DNA-tagged antibody and lectin affinity chromatography for glyco-biomarker developmentPubmed:25525205
  • Sipa1l1 is an early biomarker of liver fibrosis in CCl4-treated ratsPubmed:27230648
  • Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese PatientsPubmed: 30486327
  • Protective Activity of Taurine and Molecular Fibrogenesis in Iron Overloaded Hepatic Tissues

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