Polyclonal Antibody to Pepsin (PP) Homo sapiens (Human) Polyclonal antibody

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Pepsin (PP) Packages (Simulation)
  • Polyclonal Antibody to Pepsin (PP) Packages (Simulation)
  • Polyclonal Antibody to Pepsin (PP) DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human PP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pepsin (PP) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pepsin (PP) DAB staining on IHC-P;
    Sample: Human Prostate Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PP Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pepsin (PP) DAB staining on IHC-P;
    Sample: Human Stomach Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PP Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA632Hu01.jpg Western Blot; Sample: Lane1: Human Urine; Lane2: Porcine Stomach lysate Primary Ab: 0.05µg/ml Rabbit Anti-Human PP Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PP. It has been selected for its ability to recognize PP in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • LEVEL OF PEPSIN AND BILE ACIDS IN THE SALIVA OF PATIENTS WITH GLOTTIS T1 CARCINOMASzd:Source
  • Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancerPubmed:Pmc4362607
  • Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux – a prospective comparative studyPubMed: 25516364
  • Does Positioning Affect Tracheal Aspiration of Gastric Content in Ventilated Infants?PubMed: 25313850
  • Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakiapubmed:28756936
  • Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed ElectrodePubmed:29882917
  • Association between Pepsin in Bronchoalveolar Lavage Fluid and Prognosis of Chronic Fibrosing Interstitial Lung Disease
  • Pulmonary aspiration in preschool children with cystic fibrosisPubmed: 30558606
  • Association of Bile Acid and Pepsin Micro-aspiration with Chronic Obstructive Pulmonary Disease ExacerbationPubmed: 31423141
  • Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal RefluxPubmed: 31935973
  • Presence of pepsin in laryngeal tissue and saliva in benign and malignant neoplasmsPubmed: 33103719
  • Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord LeukoplakiaPubmed: 32692278
  • Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polypsPubmed: 32889371
  • The relationship between laryngopharyngeal reflux based on pepsin value and clinical characteristics of laryngeal cancer patients
  • The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and 24-Hour?¡­33220027
  • Pepsin properties, structure, and its accurate measurement: a narrative review
  • The Impact of Laryngopharyngeal Reflux on Occurrence and Clinical Course of Recurrent Respiratory Papillomatosis34338331
  • Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumoniaPubmed:35715334
  • Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel SyndromePubmed:35088760

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