Polyclonal Antibody to Thioredoxin (Trx) 
TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein
Overview
Properties
- Product No.PAA702Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayDevelopmental science
- SourcePolyclonal antibody preparation, Host Rabbit
- Ig Type IgG, Potency n/a
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA702Hu01-Recombinant Thioredoxin (Trx)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 500µg/mL
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Packages (Simulation)
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Packages (Simulation)
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FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Human Trx Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant Trx, Human.
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Figure. Western Blot; Sample: Lane1: Human hepG2 Cells; Lane2: Human MCF7 Cells; Lane3: Human Hela Cells.
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HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human Trx Polyclonal Antibody (Catalog # PAA702Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human Trx Polyclonal Antibody (Catalog PAA702Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a rabbit polyclonal antibody raised against Trx. It has been selected for its ability to recognize Trx in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis OxfordJournals: source
- Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosusPubmed:Pmc4061188
- Alteration in the intrafollicular thiol–redox system in infertile women with endometriosisPubmed:25376627
- Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stressPubmed:25564095
- Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung.Pubmed:26797643
- Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the …Pubmed: 30351992
- Redox, immune and genetic biomarker system for personalized treatments in colorectal cancerDoi: 10.4251/wjgo.v11.i2.117
- The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in TriathletesPubmed: 30736479
- Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitusPubmed: 31351037
- Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive EffortPubmed: 32210109
- Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ responsePubmed: 33161477
- Examination of androgenetic alopecia with serum biomarkersPubmed: 32969583
- Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats34159266
- Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat34155936