Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Rattus norvegicus (Rat) Polyclonal antibody

CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA066Ra03-Recombinant Interleukin 1 Receptor Type I (IL1R1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.34mg/ml
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  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Packages (Simulation)
  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Packages (Simulation)
  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) IHC Images
  • PAA066Ra03.jpg Figure. Western Blot; Sample: Recombinant IL1R1, Rat.
  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Western Blot; Sample: Rat Spleen lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Rat IL1R1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Western Blot; Sample: Rat Lung lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Rat IL1R1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Western Blot; Sample: Lane1: Rat Placenta lysate; Lane2: Rat Lung lysate; Lane3: Mouse Placenta lysate; Lane4: Mouse Lung lysate
    Primary Ab: 0.4µg/ml Rabbit Anti-Rat IL1R1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Western Blot; Sample: Human Raji cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Rat IL1R1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1) Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-rat IL1R1 Polyclonal Antibody (Catalog # PAA066Ra03, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IL1R1. It has been selected for its ability to recognize IL1R1 in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Mus musculus (Mouse)

Giveaways

Citations

  • In vivo extravasation induces expression of IL-1R1 in human neutrophilsWiley: source
  • The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective EndocarditisPlosone: 0031490
  • Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel diseasePubMed: 22267087
  • A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
  • IL1R1 基因多态性与儿童哮喘的相关性article:13877
  • IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathwaysPubmed: 33107399
  • Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in ratsPubmed:35643766

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