KO-Validated
Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1)

CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 95.00 US$ 221.00 US$ 316.00 US$ 790.00 US$ 3,160.00
Quantity

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Overview

Properties

Product No.PAA066Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction CD & Adhesion molecule Infection immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA066Hu01-Recombinant Interleukin 1 Receptor Type I (IL1R1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human IL1R1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant IL1R1, Human.
Western Blot; Sample: Raji cell lysate
Primary Ab: 3µg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Raji cell lysate;
Lane 2: IL1R1 knockout Raji cell lysate;
Predicted MW: 65kd
Observed MW: 65kd
Primary Ab: 3µg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human IL1R1 Polyclonal Antibody (Catalog PAA066Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IL1R1. It has been selected for its ability to recognize IL1R1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Increment services

Citations

In vivo extravasation induces expression of IL-1R1 in human neutrophilsWiley: source
The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective EndocarditisPlosone: 0031490
Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel diseasePubMed: 22267087
A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
IL1R1 基因多态性与儿童哮喘的相关性article:13877
IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathwaysPubmed: 33107399
Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in ratsPubmed:35643766

KO-Validated Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1)