Polyclonal Antibody to Cytokeratin 20 (CK 20) Homo sapiens (Human) Polyclonal antibody

KRT20; Keratin 20; Protein IT; Keratin, type I cytoskeletal 20

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB240Hu01-Recombinant Cytokeratin 20 (CK 20)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Cytokeratin 20 (CK 20) Packages (Simulation)
  • Polyclonal Antibody to Cytokeratin 20 (CK 20) Packages (Simulation)
  • Polyclonal Antibody to Cytokeratin 20 (CK 20) DAB staining on IHC-P; Samples: Human Lung Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human KRT20 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cytokeratin 20 (CK 20) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human KRT20 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB240Hu01.jpg Figure. Western Blot; Sample: Recombinant KRT20, Human.
  • Polyclonal Antibody to Cytokeratin 20 (CK 20) Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Porcine Intestine Tissue.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CK 20. It has been selected for its ability to recognize CK 20 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

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