Polyclonal Antibody to Ki-67 Protein (Ki-67) Homo sapiens (Human) Polyclonal antibody

MKI67; KIA; Ki67P; Antigen Identified By Monoclonal Antibody Ki-67

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC047Hu02-Recombinant Ki-67 Protein (Ki-67)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.58mg/ml
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  • Polyclonal Antibody to Ki-67 Protein (Ki-67) Packages (Simulation)
  • Polyclonal Antibody to Ki-67 Protein (Ki-67) Packages (Simulation)
  • Polyclonal Antibody to Ki-67 Protein (Ki-67) DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human Ki67P Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ki-67 Protein (Ki-67) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human Ki67P Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC047Hu02.jpg Figure. Western Blot; Sample: Recombinant Ki67P, Human.
  • Polyclonal Antibody to Ki-67 Protein (Ki-67) Figure. Western Blot; Sample: Lane1: Human Placenta Tissue; Lane2: Human PC-3 Cells; Lane3: Human MCF7 Cells.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against Ki-67. It has been selected for its ability to recognize Ki-67 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;1:300-1200
Immunohistochemistry: 5-20µg/mL;1:30-120
Immunocytochemistry: 5-20µg/mL;1:30-120
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Expressions and clinical significances of c-MET, p-MET and E2f-1 in human gastric carcinomaPubmed: 24393368
  • Expressions and clinical significances of c-MET, p-MET and E2f-2 in human gastric carcinomaPubmed:24393368
  • Chemical composition of aqueous‐ethanol extract of leaves and its effect on representation of caspase 8, caspase 3 and the proliferation marker Ki67 in intrinsic …Pubmed:29874411
  • Predictive value of the proliferation marker Ki-67 in patients with acute leukemia undergoing hematopoietic stem cell transplantation
  • A novel exopolysaccharide from marine bacterium Pantoea sp. YU16-S3 accelerates cutaneous wound healing through Wnt/β-catenin pathwayPubmed: 32299547

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