• KO-Validated
Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) Mus musculus (Mouse) Polyclonal antibody

CRARF; PRSS5; Mannan-Binding Lectin Serine Peptidase 1; Ra-reactive factor serine protease p100; Complement-activating component of Ra-reactive factor; Serine protease 5

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB895Mu01-Recombinant Mannose Associated Serine Protease 1 (MASP1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) Packages (Simulation)
  • Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) Packages (Simulation)
  • Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 10μg/ml Rabbit Anti-Mouse MASP1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) DAB staining on IHC-P;
    Sample: Mouse Liver Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Mouse MASP1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) DAB staining on IHC-P;
    Sample: Mouse Stomach Tissue;
    Primary Ab: 10ug/ml Rabbit Anti-Mouse MASP1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB895Mu01.jpg Figure. Western Blot; Sample: Recombinant MASP1, Mouse.
  • Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) Western Blot; Sample: Lane1: Rat Liver lysate; Lane2: Hela cell lysate
    Primary Ab: 1µg/ml Rabbit Anti-Mouse MASP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mannose Associated Serine Protease 1 (MASP1) Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: MASP1 knockout Hela cell lysate;
    Predicted MW: 80,82kDa
    Observed MW: 90kDa
    Primary Ab: 1µg/ml Rabbit Anti-Mouse MASP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MASP1. It has been selected for its ability to recognize MASP1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Increased Circulating Plasma Mannose Binding Lectin Associated Serine Protease (Masp) As A Result of Enu-Induced Mutation in Masp-1Lww: Source
  • Bisretinoid-mediated Complement Activation on Retinal Pigment Epithelial Cells Is Dependent on Complement Factor H HaplotypePubmed:24550392
  • The Levels of the Lectin Pathway Serine Protease MASP-1 and Its Complex Formation with C1 Inhibitor Are Linked to the Severity of Hereditary AngioedemaPubMed: 26371246
  • Mannose-binding lectin-associated serine protease 2 (MASP-2) contributes to poor disease outcome in humans and mice with pneumococcal meningitisPMC5234106
  • AmpliSeq screening of genes encoding the C-type lectin receptors and their signaling components reveals a common variant in MASP1 associated with …Pubmed:29515573
  • Plasma proteome profiling of high-altitude polycythemia using TMT-based quantitative proteomics approachPubmed: 30605725
  • Clinical value of complement activation biomarkers in overt diabetic nephropathy
  • Comparison of Complement Pathway Activation in Autoimmune GlomerulonephritisPubmed:35571000

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