IMMUNOGEN INFORMATIONImmunogen: Recombinant MYH6 (Glu856~Ile1116) expressed in E.coli.
Accession No.: RPD419Ra01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-EFGRV KDALEKSEAR RKELEEKMVS LLQEKNDLQL QVQAEQDNLA DAEERCDQLI KNKIQLEAKV KEMTERLEDE EEMNAELTAK KRKLEDECSE LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EEMAGLDEII AKLTKEKKAL QEAHQQALDD LQAEEDKVNT LTKSKVKLEQ QVDDLEGSLE QEKKVRMDLE RAKRKLEGDL KLTQESIMDL ENDKLQLEEK LKKKEFDISQ QNSKIEDEQA LALQLQKKLK ENQARI ANTIBODY SPECIFITY The antibody is a rabbit polyclonal antibody raised against MYH6. It has been selected for its ability to recognize MYH6 in immunohistochemical staining and western blotting. APPLICATIONS WB, ICC, IHC-P, IHC-F, ELISA
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. QUALITY CONTROL Content: The quality control contains recombinant MYH6 (Glu856~Ile1116) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.