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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
ASD3; MYHC; MYHCA; Alpha-MHC; Cardiomyopathy,Hypertrophic 1
Organism species Rattus norvegicus (Rat)
Product No.PAD419Ra01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant MYH6 (Glu856~Ile1116) expressed in E.coli.
Accession No.: RPD419Ra01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-EFGRV KDALEKSEAR RKELEEKMVS LLQEKNDLQL QVQAEQDNLA DAEERCDQLI KNKIQLEAKV KEMTERLEDE EEMNAELTAK KRKLEDECSE LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EEMAGLDEII AKLTKEKKAL QEAHQQALDD LQAEEDKVNT LTKSKVKLEQ QVDDLEGSLE QEKKVRMDLE RAKRKLEGDL KLTQESIMDL ENDKLQLEEK LKKKEFDISQ QNSKIEDEQA LALQLQKKLK ENQARI
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MYH6. It has been selected for its ability to recognize MYH6 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications
PAD419Ra01Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)WB, ICC, IHC-P, IHC-F, ELISA
PAD419Ra02Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)WB, ICC, IHC-P, IHC-F, ELISA
PAD419Ra03Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)WB, ICC, IHC-P, IHC-F, ELISA
PAD419Ra71Biotin-Linked Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)IHC;WB;ELISA
RPD419Ra01Recombinant Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)SDS-PAGE; WB; ELISA; IP.
RPD419Ra02Recombinant Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)SDS-PAGE; WB; ELISA; IP.
RPD419Ra03Recombinant Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)SDS-PAGE; WB; ELISA; IP.
SED419RaELISA Kit for Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)Enzyme-linked immunosorbent assay