Polyclonal Antibody to Cytosolic Phospholipase A2 (PLA2G4)

c-PLA2; cPLA2; PLA2G4A; Phospholipase A2, Cytosolic; Phospholipase A2,Group IV(Cytosolic,Calcium-Dependent); Phosphatidylcholine 2-acylhydrolase; Lysophospholipase

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 103.00 US$ 239.00 US$ 342.00 US$ 855.00 US$ 3,420.00
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Overview

Properties

Product No.PAB624Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Metabolic pathway Cardiovascular biology

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB624Mu01-Recombinant Cytosolic Phospholipase A2 (PLA2G4)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse PLA2G4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse PLA2G4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Western Blot; Samples: Lane1: Hela cell lysate; Lane2: NIH/3T3 cell lysate; Lane3: RAW264.7 cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Mouse PLA2G4 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PLA2G4. It has been selected for its ability to recognize PLA2G4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Citations

The synergistic mechanism of total saponins and flavonoids in Notoginseng− Safflower pair against myocardial ischemia uncovered by an integrated …Pubmed: 32739736