• KO-Validated
Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Homo sapiens (Human) Polyclonal antibody

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA588Hu01-Recombinant Pyruvate kinase isozymes M2 (PKM2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Packages (Simulation)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Packages (Simulation)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P; Samples: Human Breast cancer Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Skin cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Sample: Mouse Lung Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Prostate Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Prostate cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Sample: Mouse Testis Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human PKM2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) FITC staining on IF;
    Samples: Human MCF7 cell;
    Primary Ab: 20ug/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) AF488 staining on IF;
    Sample: Hela cell
    Primary Ab: 20µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAA588Hu01.jpg Figure. Western Blot; Sample: Recombinant PKM2, Human.
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: PKM2 knockout MCF7 cell lysate;
    Predicted MW: 56,58kd
    Observed MW: 60kd
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PKM2 Polyclonal Antibody (Catalog PAA588Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PKM2 Polyclonal Antibody (Catalog PAA588Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;

Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3AACR: 70189
  • Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancerPubmed:25483567
  • M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cellsPubMed: 26404132
  • Identification of a serum biomarker panel for the differential diagnosis of cholangiocarcinoma and primary sclerosing cholangitisPubmed:29707118
  • Acteoside improves muscle atrophy and motor function by inducing new myokine secretion in chronic spinal cord injuryPubmed: 30318996
  • Secreted Pyruvate Kinase M2 Promotes Lung Cancer Metastasis through Activating the Integrin Beta1/FAK Signaling PathwayPubmed: 32049010
  • Chrysin serves as a novel inhibitor of DGKα/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC)
  • Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins
  • Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression34376212
  • Putative Association between Low Baseline Gene Expression in the Peripheral Blood and Clinical Remission in Rheumatoid Arthritis Patients Treated with Tofacitinib34947916

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